1,175 research outputs found

    Effective Brief-Writing for California Appellate Courts

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    This Article suggests an approach to appellate brief writing that will insure compliance with the rules of court and a clear expression of the advocate\u27s position in the issues. The author describes both the specific techniques of effective brief writing and the technical format used in writing briefs for California appellate courts. Providing practical tools and techniques for the practitioner, the author argues that adherence to these techniques will significantly improve the quality of appellate practice in California

    Boundedness properties of fermionic operators

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    The fermionic second quantization operator dΓ(B)d\Gamma(B) is shown to be bounded by a power Ns/2N^{s/2} of the number operator NN given that the operator BB belongs to the rr-th von Neumann-Schatten class, s=2(r1)/rs=2(r-1)/r. Conversely, number operator estimates for dΓ(B)d\Gamma(B) imply von Neumann-Schatten conditions on BB. Quadratic creation and annihilation operators are treated as well.Comment: 15 page

    Identification of circulating parasite antigen in patients with bancroftian filariasis

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    Because many cases of lymphatic filariasis cannot be diagnosed either clinically or by immunodiagnostic test based on antibody detection, recent efforts have been more directed towards developing methods for detecting parasite antigen in the blood or urine. Using a solid phase (Sepharose 4B) two-site immunoradiometric assay (IRMA) employing hyperimmune rabbit antifilarial antisera, we have previously shown (Hamilton et al., 1984) that essentially all cases ofpatent (ie. microfilaremic) infection in patients with bancroftian filariasis can be detected by this semi-quantitative assay as well as some individuals with amicrofilaremic (i.e., 'cryptic') infection. The present communication reports the results of studies that identify a prominent circulating antigen detected by this IRMA in sera from patients with microfilaremia. The antigen was eluted from Sepharosebound rabbit polyclonal antiserum that had been reacted with known antigen positive sera. It was run in SDS-PAGE, blotted to nitrocellulose paper and identified autoradiographically using '25l-labelled rabbit antifilarial antiserum. Its high molecular weight (- 200 kD), stability to acid and boiling, and sensitivity to pronase and periodate suggest its being a glycoprotein. Isolation of this antigen will permit the development of specific reagents (such as monoclonal antibodies) which should enhance both the sensitivity and utility of the currently available antigen detection systems

    Variations in Postprandial Blood Glucose Responses and Satiety after Intake of Three Types of Bread

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    Background. The magnitude and duration of postprandial blood glucose (PPG) elevations are important risk factors of diabetes and coronary heart diseases. Aim. To study PPG after ingestion of breads with and without pea fibre and rapeseed oil. Methods. After fasting overnight, 10 Pakistani immigrant women participated in three experiments having a crossover design and involving ingestion of various types of bread: regular coarse bread or fibre enriched-bread with two levels of rapeseed oil, all providing 25 g available carbohydrates (CHO). Blood glucose and satiety were determined before the meal and every 15 min over the next 2 hours. Results. Intake of an amount of pea fibre-enriched bread containing 25 g CHO attenuated, the postprandial peak glucose value, the incremental area under the glucose versus time curve during 15 to 75 min, and the glycemic profile, and increased duration of satiety (P < .05), as compared with intake of regular bread with 25 g carbohydrate. Conclusion. Pea fibre-enriched breads can reduce PPG and prolong satiety

    Bacterial community diversity and variation in spray water sources and the tomato fruit surface

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    <p>Abstract</p> <p>Background</p> <p>Tomato (<it>Solanum lycopersicum</it>) consumption has been one of the most common causes of produce-associated salmonellosis in the United States. Contamination may originate from animal waste, insects, soil or water. Current guidelines for fresh tomato production recommend the use of potable water for applications coming in direct contact with the fruit, but due to high demand, water from other sources is frequently used. We sought to describe the overall bacterial diversity on the surface of tomato fruit and the effect of two different water sources (ground and surface water) when used for direct crop applications by generating a 454-pyrosequencing 16S rRNA dataset of these different environments. This study represents the first in depth characterization of bacterial communities in the tomato fruit surface and the water sources commonly used in commercial vegetable production.</p> <p>Results</p> <p>The two water sources tested had a significantly different bacterial composition. Proteobacteria was predominant in groundwater samples, whereas in the significantly more diverse surface water, abundant phyla also included Firmicutes, Actinobacteria and Verrucomicrobia. The fruit surface bacterial communities on tomatoes sprayed with both water sources could not be differentiated using various statistical methods. Both fruit surface environments had a high representation of Gammaproteobacteria, and within this class the genera <it>Pantoea </it>and <it>Enterobacter </it>were the most abundant.</p> <p>Conclusions</p> <p>Despite the major differences observed in the bacterial composition of ground and surface water, the season long use of these very different water sources did not have a significant impact on the bacterial composition of the tomato fruit surface. This study has provided the first next-generation sequencing database describing the bacterial communities living in the fruit surface of a tomato crop under two different spray water regimes, and therefore represents an important step forward towards the development of science-based metrics for Good Agricultural Practices.</p

    Applications of Canonical Transformations

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    Canonical transformations are defined and discussed along with the exponential, the coherent and the ultracoherent vectors. It is shown that the single-mode and the nn-mode squeezing operators are elements of the group of canonical transformations. An application of canonical transformations is made, in the context of open quantum systems, by studying the effect of squeezing of the bath on the decoherence properties of the system. Two cases are analyzed. In the first case the bath consists of a massless bosonic field with the bath reference states being the squeezed vacuum states and squeezed thermal states while in the second case a system consisting of a harmonic oscillator interacting with a bath of harmonic oscillators is analyzed with the bath being initially in a squeezed thermal state.Comment: 14 page

    Characterization of microflora in Latin-style cheeses by next-generation sequencing technology

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    Background Cheese contamination can occur at numerous stages in the manufacturing process including the use of improperly pasteurized or raw milk. Of concern is the potential contamination by Listeria monocytogenes and other pathogenic bacteria that find the high moisture levels and moderate pH of popular Latin-style cheeses like queso fresco a hospitable environment. In the investigation of a foodborne outbreak, samples typically undergo enrichment in broth for 24 hours followed by selective agar plating to isolate bacterial colonies for confirmatory testing. The broth enrichment step may also enable background microflora to proliferate, which can confound subsequent analysis if not inhibited by effective broth or agar additives. We used 16S rRNA gene sequencing to provide a preliminary survey of bacterial species associated with three brands of Latin-style cheeses after 24-hour broth enrichment. Results Brand A showed a greater diversity than the other two cheese brands (Brands B and C) at nearly every taxonomic level except phylum. Brand B showed the least diversity and was dominated by a single bacterial taxon, Exiguobacterium, not previously reported in cheese. This genus was also found in Brand C, although Lactococcus was prominent, an expected finding since this bacteria belongs to the group of lactic acid bacteria (LAB) commonly found in fermented foods. Conclusions The contrasting diversity observed in Latin-style cheese was surprising, demonstrating that despite similarity of cheese type, raw materials and cheese making conditions appear to play a critical role in the microflora composition of the final product. The high bacterial diversity associated with Brand A suggests it may have been prepared with raw materials of high bacterial diversity or influenced by the ecology of the processing environment. Additionally, the presence ofExiguobacterium in high proportions (96%) in Brand B and, to a lesser extent, Brand C (46%), may have been influenced by the enrichment process. This study is the first to define Latin-style cheese microflora using Next-Generation Sequencing. These valuable preliminary data will direct selective tailoring of agar formulations to improve culture-based detection of pathogens in Latin-style cheese

    Natural disciplinarians or learning from the job? The first two years of seven male teachers in Icelandic compulsory schools

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    Funding Information: The study is supported by the Icelandic Gender Equality Fund and the Research Fund of the University of Iceland. Sunna K. Símonardóttir PhD assisted the authors with studying the research literature about male teachers. Publisher Copyright: © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.This article presents an interview study with seven newlygraduated male teachers in Icelandic compulsory schools. We interviewed them five times during their first two years of teaching. The focus is on the ways in which the gender of the novice teachers mattered in the expectations that they experienced and how these expectations interacted with the professional demands of being a teacher. The interviews reveal that hegemonic masculinity ideas have an impact on the minds of our interviewees as they experienced various expectations, based in such masculinity. The findings also suggest a tension between the expectations of men as natural disciplinarians and the professional induction of learning to become a teacher. Male-specific expectations included that the school as well as parents expected that the students had respect for them on the grounds that they were men. While such expectations gave some a head start with positional authority, it laid a burden on them as novices. Not all of our interviewees fitted the male-specific expectations, which supports the importance of breaking down gendered stereotypes. In recruiting teachers, regardless of gender, we need individuals able to perform professional practices of care and attention to detail in managing a classroom.Peer reviewe

    Multispecies diel transcriptional oscillations in open ocean heterotrophic bacterial assemblages

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    Oscillating diurnal rhythms of gene transcription, metabolic activity, and behavior are found in all three domains of life. However, diel cycles in naturally occurring heterotrophic bacteria and archaea have rarely been observed. Here, we report time-resolved whole-genome transcriptome profiles of multiple, naturally occurring oceanic bacterial populations sampled in situ over 3 days. As anticipated, the cyanobacterial transcriptome exhibited pronounced diel periodicity. Unexpectedly, several different heterotrophic bacterioplankton groups also displayed diel cycling in many of their gene transcripts. Furthermore, diel oscillations in different heterotrophic bacterial groups suggested population-specific timing of peak transcript expression in a variety of metabolic gene suites. These staggered multispecies waves of diel gene transcription may influence both the tempo and the mode of matter and energy transformation in the sea.Gordon and Betty Moore Foundation (GBMF 492.01)Gordon and Betty Moore Foundation (GBMF 3777)National Science Foundation (U.S.) (Grant EF0424599)David & Lucile Packard Foundatio
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